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Kraken2 github The rsync script should be in a similar location. I've noticed that there's a read classified as taxid 1 in both the fastq_1 and fastq_2 files. kraken2-build --build --db nt --threads 16 'Estimated hash table requirement: 345259953004 bytes' Taxonomy parsed and converted. The memory-mapping option has made it possible for me to analyze my data using Kraken2 and the NCBI NT database, however I am still stuck being unable to build a Bracken database (due to RAM limitation) from the Kraken2 NCBI NT database. tar. fastp - filter and trim reads with default parameters; kraken2 - taxonomic assignment of the reads; bracken - abundance estimation at a single level in the taxonomic tree, e. I downloaded kraken2-microbial-fatfree pre-built kraken db from Loman lab. Instant dev environments #Scripts to create Kraken2 database of I started using Kraken2 and I tried to classified some reads and separate classified and unclassified reads with --classified-out and --unclassified-out options. Sign up for GitHub combined. Sign up for a free GitHub account to open an issue and contact its maintainers and the community. Sign up for a free GitHub account to open an issue and contact its maintainers and GitHub is where people build software. I have been using Kraken2 to classify my 16S reads with the Silva and Greengenes databases provided, which works great. /Kraken2_Database. I just had a quick look, and it looks like the issue is that the reference human genome is a 'Chromosome' rather than 'Complete' - I've I also need to find a way to preload database(s) into memory or swap. For a set of fastq files it executes:. Sign in Product GitHub Copilot. But How can I extract the classified reads of a specific taxonomic group for the followi You signed in with another tab or window. It is a great tool but it is limited to build only Kraken2 database. Kraken2 is the newest version of Kraken, a taxonomic classification system using exact k-mer matches to achieve high accuracy and fast classification speeds. pipeline nextflow Hello, I'm currently working with kraken2 using a dataset downloaded from NCBI (ERR4082713). The material I have used Kraken2 to analyze previously came from metagenomic sources and it is clear that we frequently get reads mapping to sister taxa when the target You signed in with another tab or window. Notifications You must be signed in to change notification settings; Fork 275; Star 740. 8_beta. To do that, I made my own names. The second version of the Kraken taxonomic sequence classification system - Home · DerrickWood/kraken2 Wiki Kraken taxonomic sequence classification system. The second version of the Kraken taxonomic sequence classification system - kraken2/LICENSE at master · DerrickWood/kraken2 The second version of the Kraken taxonomic sequence classification system - kraken2/scripts/kraken2 at master · DerrickWood/kraken2 I am attempting to install Kraken2 onto my linux system into the folIowing directory /usr/local/bin/kraken2 have both the "install_kraken2. Here is the code I used : kraken2-build --standard --use-ftp --no-masking --threads 24 --db dbkraken And here's the output: Step 1/2: Performing ftp Hi Nick, No worries! Glad someone else can make use of it. Enterprise-grade AI features Kraken2 is a new development that enables using smaller databases than Kraken 1 or KrakenUniq. Whatever I did kraken2 always loads the databases into memory first. 3 . I am using Kraken2 for classifying 16s amplicon data (I have around 100 samples). Find and fix vulnerabilities Sign up for a I tried downloading the viral database again today and it worked! Anyone having trouble maybe just try again code used: kraken2-build --download-library viral --threads 32 --db kraken_viral Hi there, kraken2-build download searches for the fasta file of the particular db one wants to download. Learn how to download and use Kraken 2 source Kraken2 is a program for taxonomic sequence classification. You signed out in another tab or window. com/DerrickWood/kraken2/blob/master/docs/MANUAL. sh were last edited 2 months ago, so I also tried to replace these scripts in my conda installation. However, I have used these files on SqueezeMeta without a problem. For more information, see the Kraken2 github, and/or the protocol paper describing use of Kraken2. Write better code with AI Security. To do this, run scripts/improve_taxonomy. Remarkably, Kun-peng’s processing times were comparable to Kraken2 using the standard database (81GB), roughly 5% of the size of the pan-domain database. 1. Is there something I missed before downloading the database? (I installed Kraken2 using bioconda) Hello, I have been trying to follow the steps to generate a kraken2 db of all bacteria. If you've already downloaded all of them a Kraken2 builds a database consisting of a k-mer and all the genomes that contain this k-mer. Skip to content. This classifier matches each Kraken2 is a tool which allows you to classify sequences from a fastq file against a database of organisms. k2 is using python script which is slightly slower. But when I learned that memory-mapping should use mmap function, and the software itself will handle it. Feature highlights: Supports Kraken2 or KrakenUniq for read classification. Find publications, downloads, manual, FAQ, and related tools on the GitHub wiki page. I have successfully built the SILVA database. The database consists of a list of kmers and the mapping of kraken2-build is a tool provided by Kraken2 to build Kraken2 database. Sign up Product Actions. gz inside the directory and the pwd is also this same di More than 100 million people use GitHub to discover, fork, and contribute to over 420 million projects. I have 21 new genomes (in fasta format) that I want to add to my kraken2 database. To evaluate the correlation between the size of input data and time consumed, I splitted the orignal data to 50 parts (total line number of each parts % 4 = 0). For beta diversity you have to transform your data in an OTU table like. Hi I wonder how to use minikraken_8GB_20200312_genomes. Projects None yet Milestone No milestone Development No branches or pull requests. 3. 7_beta and 2. fastp - filter and trim reads with default parameters; kraken2 - taxonomic assignment Saved searches Use saved searches to filter your results more quickly Kraken output generates a report for each datasets, this script takes these individual output and combines it to one file, where each column is number of reads that were rooted to this taxon (colum In the meantime, I found installing the kraken2 through conda is only limited to version 2. Learn how to use Kraken2, a taxonomic sequence classifier that assigns taxonomic labels to DNA sequences. Sign up for GitHub Following this guide: https://github. br I tried to re-run my pipeline which is using Kraken2 (I ran it as near as last week on the same data and it was working fine), however I got a following error: libexec/classify: invalid option -- 'H' As you can see below, my command does Hi, I am running kraken2 on marine biofilm samples, and I haven't been able to return more than 3% of sequences classified. 78-94. Kraken 2 is a tool for fast and accurate assignment of metagenomic reads to taxonomic groups. Assignees No one assigned Labels download issue kraken2-build --download-library issue. I could not run any of the available conda builds for version 2. Step 1/2: Performing ftp file transfer of requested files Creates a simple OTU table from Kraken2 report. Contribute to sipost1/kraken2OTUtable development by creating an account on GitHub. If you publish data using Learn how to build and use a custom kraken2 database for metagenomics analysis of nematode RNAseq data. Learn how to use it from the Operating Manual, the paper, and the protocol paper on GitHub. I've downloaded files from kraken2-build --download-library fungi --db fungi But when I performed kraken2-build --build --db fungi it appears an error: Can't find taxonomy/ subdirec Converting Kraken2 and Bracken output into phyloseq object - SumeetTiwari07/KBKen More than 100 million people use GitHub to discover, fork, and contribute to over 330 million projects. Dear authors, I am trying to build the standard kraken database but it failed each time. kraken2 Updated May 2, 2022; Python; savytskanatalia / Kraken2. Thank you for responding Jennifer! I tried --use-ftp with kraken2 version 2. Automate any workflow Create, add, merge different taxonomy sources (QIIME, GTDB, NCBI and more) and create metagenomic databases (kraken2, ganon and more ) ncbi-taxonomy taxonomic Hello, I'm working with a metagenome and I'm not sure what is better if using kraken2 with the output from the MEGAHIT (contigs) or using reads (paired). But at least I got some results that can be used. dmp and nodes. txt with minikraken_8GB_202003. gz inside the directory and the pwd is also this Sign up for a free GitHub account to open an issue and contact its maintainers and the community. Here's what I have tried: -Multiple databases, including kraken2 standard, kraken2 standard 8gb, RefSeqCompleteV2 download issue kraken2-build --download-library issue. Dear Developer, The Kraken2 give the results of classified sequence and taxonomic ID. Though at first it was a ressource thing, but when I run ressource monitor, some samples just crash in the middle of the library being loaded into ram while other do not (so when still half of ressources are free). More than 100 million people use GitHub to discover, fork, and contribute to over 420 million projects. Sign up for Kraken2 is a taxonomic sequence classification system. Hello! Same issue here. I installed kraken2 via conda. Is this normal? I am unsure of the up and down speeds but I am working on a HPC so it is unlikely that is the issue. I download the taxonomy and the library. Toggle navigation. I saw on github that the scripts rsync_from_ncbi. You signed in with another tab or window. The build should work after a couple days unfortunately. Contribute to DerrickWood/kraken development by creating an account on GitHub. pl and download_genomic_library. g. I recall @jenniferlu717 said there was a minikraken2 database being made available at some point? Any update on this? Would help a lot to get people installing tools dependent on kraken2. AI-powered developer platform Available add-ons. py with the database directory as the only The second version of the Kraken taxonomic sequence classification system - kraken2/data/README. 27 Gbp) done. Kun-peng classified 69. kmer_distrib ├── database150mers. Enterprise-grade security features GitHub Copilot. Since the length of the sequences @MichaelAngelou I also wanted to do alpha and beta diversity but it turns out that alpha diversity is quite codified for species level/OTU so you can hardly do it with kraken output but for beta diversity it is just fine ! There must be some ways of transforming he data for alpha diversity but I don't know how. pl: unexpected FTP path (new server?) for https:/ Hi Jen, The database we use is the standard Kraken 2 database (which, per the website, contains "NCBI taxonomic information, as well as the complete genomes in RefSeq for the bacterial, archaeal, and viral domains, along with the human genome and a collection of known vectors (UniVec_Core)”). . The data processed in my work is single-ended sequencing data. As suc Using the standard 16gb library and some bacterial reads we seem to get empty taxonomic level fields in the kraken2 report file. Kraken 2 is a rewrite of Kraken 1 and a tool for classifying metagenomic sequences. Instant dev environments GitHub community articles The code will run and seems to be able to find the files I need, but says there are 0 sequences. It is possible that the process can get This happens when NCBI hasnt updated their assembly_summary. kmer_distrib A Kraken2 + Bracken based pipeline for classifying and quantifying microbial reads from GDC TCGA WGS data. For example, the root and bacteria lines have no taxonomic level. Topics Trending Collections Enterprise Enterprise platform. I'm struggling because the contigs are large (min length is 1000 bp) and I don't kn You signed in with another tab or window. GitHub is where people build software. Browse the latest releases, bug fixes, features, and download links on GitHub. Find out the system requirements, installation, database structure, command line options, output formats, and more. I have done this before with no problems but today I am getting errors - it seems others are too. Hence I tried with kraken2 (f ##set up conda env #mamba create -n kraken2 -c bioconda -c conda-forge kraken2 conda activate kraken2 ##the most difficult and important step with kraken is the database of kmers to be used ##these databases are built using NCBI sequences and taxonomic assignments ##there is a standard databases but it is purely designed for human data ##therefore we need to create You signed in with another tab or window. gz compression is the issue since they're all zipped, and some work while others don't, but I'll try --gzip-compressed and see if that makes the difference! Dear All, We have done V1-V9 illunima sequencing for our amplicon sequence analysis. kraken2-bui The second version of the Kraken taxonomic sequence classification system - DerrickWood/kraken2. I already have few other changes in my repo. However, the cat bacteriadb archaeadb won't work because the databases (bacteriadb, archaeadb etc) are directories. These genomes do not exist in the NCBI taxonomy. tsv file, which contains an improved version of the ncbi taxonomy for downstream analysis. And using the command: kraken2-build --build --db fungi_refseq200731/ --threads 18 I get the following error: Sequence ID to taxonomy ID map al The download code already had the HTTPS on line. txt file that the k2mask will reference the next time the masking process begins. I was able to rebuild the database Kraken output generates a report for each datasets, this script takes these individual output and combines it to one file, where each column is number of reads that were rooted to this taxon (column 2 in summary) - npbhavya/Kraken2-output-manipulation Same question occured to me recently. Sign in Product a simple python script for "grafting" on novel sequences to a custom Kraken2 database. Sign in Product Actions. Instant dev environments For issues with any of the listed tools, Dear developer, I'm trying to build the fungi database. Write better code with AI Create, add, merge different taxonomy sources (QIIME, GTDB, NCBI and more) and create metagenomic databases (kraken2, ganon and more ) ncbi I am attempting to install Kraken2 onto my linux system into the folIowing directory /usr/local/bin/kraken2 have both the "install_kraken2. This process is not 100% fullproof though. The pipeline runs in a docker container by default. Automate any workflow Codespaces. DerrickWood / kraken2 Public. I built a custom db by using the following command kraken2-build --download-taxonomy --db refseq kraken2-build --down Simple Kraken2 Nextflow pipeline. Find and fix vulnerabilities A simple nextflow pipeline for running kraken2 and bracken in a docker container. 1 Hello, I ran into a similar issue not too long ago while building NT. I run kraken2 with --memory-mapping while put database on disk. I not A Kraken2 downstream analysis toolkit. Contribute to danisven/KrakMeOpen development by creating an account on GitHub. If you don't have admin permissions, you might be able to install kraken2 in your local directory and modify the script from there. This database, built using a user-specified library of genomes, allows a quick lookup of the most specific node in the taxonomic I am trying to build the kraken nt database with 5 threads and 20gb of memory but it is taking over 5 hours. A failed masked attempt will create a masked_sequences. Here is the code I used : kraken2-build --standard --use-ftp --no-masking --threads 24 --db dbkraken And here's the output: Sign up for a free GitHub account to open an issue and contact its maintainers and the community. The classified reads looks like this: fastq_1: @ER You signed in with another tab or window. But my folder is structured as follows: k2_standard_20240112 ├── database100mers. Kraken version 2. Contribute to maxibor/kraken-nf development by creating an account on GitHub. I am using 72Gb standart DB. dmp files following the same logic that it was uses for the 16S databases. 5GB) 8GB Kraken 2 Database built from the refseq bacteria, archaea, and viral libraries. However, Hi, I'm trying to build a custom database for Fungi. 0. Then I commanded kraken2-build --build --db ${DBNAME} and built this database. Contribute to SilasK/Krak development by creating an account on GitHub. txt file to remove/change links that no longer work. It seems there is something wrong with the recent conda builds of kraken2. species, using the kraken2 output; kaiju - taxonomic The second version of the Kraken taxonomic sequence classification system - DerrickWood/kraken2. Sign in Product Sign up for a free GitHub account to open an issue Kraken 2 is one of the most popular tools used for assigning taxonomic labels to short DNA sequences. Next, to generate bracken k-mer distribute file, I need seqid2taxid. This is useful when looking for a species of interest or contamination. Ive executed standard QIIME2 pipeline using GG database. And yes that looks correct. It is very large (I think it's now close to 1TB in size) and will use a lot of RAM to load into memory when running Kraken2. kdb is a more generic tool which can be used to build databases for Kraken2, Centrifuge, Bracken, etc. What you can do then is make a combined "paired" file by concatenating the reads with an "x" in the middle and then just run: Hi:) I ran kraken2. But when I loop it to do my 100 samples, the console command just close by itself after exactly 4 samples. tgz, if it has to be used with it. MiniKraken2_v1_8GB: (5. However, I wanted to know about processing multiple samples. Instant dev environments GitHub community articles The pipeline runs in a docker container by default. If you're not in the Bhatt lab or are using a new database, build it with instructions from the Kraken2 github or the custom instructions below. Would help a lot to get people installing tools dependent on kraken2. Contribute to liu930724/meta_profile development by creating an account on GitHub. Navigation Menu Toggle navigation. following is the things I see on my page once I apply the command. Perhaps that will help? The second version of the Kraken taxonomic sequence classification system - kraken2/data/README. In all other steps, performance of k2 is at par with kraken2-build. So it has to be used with the Kraken2 --memory-mapping option. I ran 'kraken2-build --db krakendb --download-taxonomy' and created a database with bacteria data (kraken2-build --db krakendb --download-library bacteria), when I tried to build the database, it throw me 'No preliminary seqid/taxid mapp Snakemake for flexitaxd and Kraken2. GitHub community articles Repositories. Already have an account? Sign in to comment. 1 and installed then used --use-ftp flag while building and this time it started to build but while processing it was giving this: After that, running kraken2 with --memory-mapping options became very efficient, every task finished within 1 min. Here is the command I launched: kraken2 --db ${KRAKEN2_DB} (Kraken) [AB-Kraken2]$ kraken2-build --download-library bacteria --db Try1 Step 1/2: Performing rsync file transfer of requested files Rsync file transfer complete. I have augmented k2mask with the ability to resume a failed masking attempt. Find and fix vulnerabilities Actions. If you download them separately, then you need to build each of them separately. Can I process all the samples in a single run or will I need to run Kraken2 multiple times (one sample at a time). Hi, Like others previously, I would like to use kraken2 on multiple sets of reads without having to wait to load the database each time. mpa: combined kraken2 report in mpa (MetaPhlAn) format; combined_bracken: contains the combined bracken outputs (one file for each taxonomic level, from Domain D to Species S); combine_bracken_reports: same as combined_bracken but in kraken2 report format; When I run Kraken2 on a single file, no problems. 8 and it did not work so I read other issues and figured out that was a version issue so I downloaded latest kraken2 version which is 2. You switched accounts on another tab or window. Thanks, Ankit Metaphlan3, Humann3, Kraken2+Bracken, UniAlign. md at master · DerrickWood/kraken2 I have same issue. Follow the steps to download taxonomy data, prepare genomes, and run kraken2 commands. Skip to content Toggle navigation. Sign up for GitHub By clicking “Sign up for GitHub Hi, I tried to run the command kraken2-build --db krakendb --build but this is the output I get: I tried to change the treads and still ran into this issue. kraken2-build --build --db nt --threads 32 Creating sequence ID to taxonomy ID map (step 1) Hello I'd like to build the "plasmid" database. 2 for my mice gut microbiome shotgun metagenomic analysis. 29% of reads, achieving 38-43% higher classification rates than Kraken2 with the standard database. When I say crash, I mean that the command windows just closes by itself. From April'24, NCBI only provides the fasta file for a few dbs, (Nt & nr) "In April 2024, the BLAST FASTA files in this directory wi All I am saying that you should not link to data that does not exist. kraken2. sh" and the source code kraken2-2. Actually it crashed the entire server after ~6 days. I downloaded the You signed in with another tab or window. Currently, with default parameters the results are not bad as such but the proportion of classification is not accurate (saying t Thanks I will try the new loop. 7-beta Star 0 Where I work we have a prebuilt NT database for kraken2. The second version of the Kraken taxonomic sequence classification system - DerrickWood/kraken2 Many taxa are not represented in the plants genomes database (that I think Kraken2 uses for the 'plants' database build). Hi, I am classifying RNA-Seq paired end reads from a fungus using the standard db (Ben Langmead Index zone) which covers archaea, bacteria, viral, plasmid, human, UniVec_Core. Hello, I am making a new Kraken database that contain many genomes not in RefSeq. 7 but by using that version the problem was solved Still wondering why. would you please help me to find where the problem is? Here is my code: time kraken2 --db ~/db/kraken2 --paired te Snakemake workflow for metagenomic classification with Kraken2 - bhattlab/kraken2_classification You signed in with another tab or window. Crea The second version of the Kraken taxonomic sequence classification system - kraken2/scripts/k2 at master · DerrickWood/kraken2 I've got the --paired flag, and I don't think the . This project builds on the classification functionality to provide a server-client architecture to allow two use cases: Access to the classification algorithms in low-resource settings by I also need to find a way to preload database(s) into memory or swap. Would you be able to share the threshold you use for this approach? Do y This is a kraken1 issue and unfortunately, we no longer support kraken 1. md at master · DerrickWood/kraken2 More than 100 million people use GitHub to discover, fork, and contribute to over 420 million projects. map files for the kraken2-microbial-fatfree database You signed in with another tab or window. However, I got the following output. The metagenomic sequences are broken down into k-mers, and each k-mer is queried against the Kraken2-built k-mer database to classify the The second version of the Kraken taxonomic sequence classification system - DerrickWood/kraken2 Hi is there any solution to this problem, when i run this command line kraken2-build --build --db database it prompts: Creating sequence ID to taxonomy ID map (step 1) No preliminary seqid/taxid mapping files found, aborting. Reload to refresh your session. ALL the download links that you feature of the manual page (!) are invalid. Automatically builds the latest MicrobialDB nucleotide and protein Kraken2 databases (for KrakenUniq nucleotide only). Just wanted to share in case anyone else encounters the same issue. markdown#custom-databases As a test, I'm trying out sequence adding to an I ran 'kraken2-build --db krakendb --download-taxonomy' and created a database with bacteria data (kraken2-build --db krakendb --download-library bacteria), when I tried to build the database, it throw me 'No preliminary seqid/taxid mapp Dear authors, I am trying to build the standard kraken database but it failed each time. For the download of taxonomy i used: kraken2-build --download-taxonomy --db database I have Kraken2 installed with conda and I am trying to build a database. Dear @jenniferlu717 and team, I read in the Kraken2 FAQ that you prefer to keep read assigned with low confidence and filter using the minimizer statistics instead. `rsync_from_ncbi. I am using More than 100 million people use GitHub to discover, fork, and contribute to over 420 million projects. The only other thing you need to do is create the taxonomy_array. But in qiime2 results, i didnt get any species level information. The second version of the Kraken taxonomic sequence classification system - DerrickWood/kraken2. From what I can piece together, this is possible using the memory mapping option. I downloaded "plasmid" library using --download-library TYPE option. My dataset consists of all sequences with a length of 31 pb, and the length of the k-mers is also set to 31. Hi, When I run my kraken2 script, it only generated an output file, but not a report file which I needed in the same time. Code; Issues 469; Pull New issue Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community. report: combined kraken2 report; combined. A report including aggregrate counts/clade also was generated. but when I apply this command only one "taxonomy" folder is created and the folder is empty. Both Illumina and Nanopore data can be processed (separately). (It only contains 3 k2d files, there is no seqid2taxid file). I would expect reads to map to other members of the Phylum. [B^[[B Currently kraken2-build is using shell commands for sequence to tax id map. Automate any workflow Create, add, merge different taxonomy sources (QIIME, GTDB, NCBI and more) and create metagenomic databases (kraken2, ganon and more ) ncbi-taxonomy Hi everyone, I'm trying to understand how to set the confidence value for my Kraken2 analysis. Comments. I have downloaded the 'nt' database, and I'm attempting to build the database using the following command: kraken2-build --db krakendb --build --threads 32 I have also tried setting the virtual memory limit using: ulimit -v 80000000 and I'm not sure exactly how you want to combine everything, but if this helps, the way that Kraken2 processes paired reads is by first concatenating them with "x" and then just running them the usual way. And now I get this message while doing the standard build. Hi, I think I'm on the exactly same issue. Hi @rbtoscan, I'm no expert. However, Kraken2 does You signed in with another tab or window. At the core of Kraken is a database that contains records consisting of a k-mer and the LCA of all organisms whose genomes contain that k-mer. Kraken2 does not use jellyfish for kmer counting. Kraken 2 is a tool for fast and accurate metagenomic sequence classification. Advanced Security. The second version of the Kraken taxonomic sequence classification system - kraken2/docs/Makefile at master · DerrickWood/kraken2 You signed in with another tab or window. I would like to compare the results to NCBI's standard 16S database as well. The second version of the Kraken taxonomic sequence classification system - Publications · DerrickWood/kraken2 Wiki Once you load the module, you can run "which kraken2" to find where it has been installed. Copy link Sign up for free to join this conversation on GitHub. Instant dev environments GitHub community articles Hi all, First, I wanted to thank the kraken2 team for making a wonderful tool for open source use. kraken2-build --standard --use-ftp --db . Host and manage packages Security. Anyway, to build the NT database they have to use the --fast-build option. The task runs very very slow, not finished after two hour. Automate any workflow Packages. --memory-mapping is taking a long long time no luxury to use it . This could be an issue caused by updates in jellyfish that we did not account for in the kraken code as we stopped updating kraken 1. Build code now creates databases with deterministic MD5 sums by default; --fast-build option to kraken2-build introduced to access old behavior Added plasmid library to standard installation set Updated SILVA to release 138. Supports HISAT2 or Bowtie2 for host filtering. Step 2/2: Assigning taxonomic IDs to sequences Processed 29372 projects (69219 sequences, 122. Only this step can be delegated to a shell script which will improve k2 build performance. Kraken 2 is a fast and accurate tool for metagenomic sequence classification using exact k-mer matches. I've run the commands kraken2-build --threads 8 --download-taxonomy --db all_bac kraken2-build --download Skip to content. The report indicates a considerable part of 'other sequences' Hello, I am planning to dig deep in my nanopore shotgun data and figure out how i can make better analysis. Learn about its features, improvements, publications, and Find links to Kraken 2 indices hosted on Amazon Web Services and GitHub, as well as pre-built databases for metagenome classification. rzsu ikgvo jpghi zezjh dirq cyscdmp rpblk titke bkoafvpo fvtlq